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dna microarray analysis nuip on chip cells  (Thermo Fisher)


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    Thermo Fisher dna microarray analysis nuip on chip cells
    Dna Microarray Analysis Nuip On Chip Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+microarray+chip/Trypsin/pmc03736349-87-3-19
    Average 99 stars, based on 1 article reviews
    dna microarray analysis nuip on chip cells - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (#Hs01042019_g1) and TaqMan® Fast Universal PCR Master Mix (Applied Biosystems; #4352042). ..

    Reverse Transcription:

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (#Hs01042019_g1) and TaqMan® Fast Universal PCR Master Mix (Applied Biosystems; #4352042). ..

    Random Hexamer:

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (#Hs01042019_g1) and TaqMan® Fast Universal PCR Master Mix (Applied Biosystems; #4352042). ..

    Gene Expression:

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (#Hs01042019_g1) and TaqMan® Fast Universal PCR Master Mix (Applied Biosystems; #4352042). ..

    Article Title: Virus-induced RGMa expression drives neurodegeneration in HTLV-1–associated myelopathy
    Article Snippet: The isolated RNA underwent reverse transcriptase (RT) reactions using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO) following the manufacturer’s protocol. .. Real-time qPCR was conducted using ABI Prism 7500 SDS (Applied Biosystems), FastStart Universal Probe Master (ROX) (Roche Diagnostics), and human FAM-labeled TaqMan gene expression primers for the following genes: RGMA (Hs00297192_m1, Applied Biosystems) and Eukaryotic 18S rRNA (4319413E, Applied Biosystems). .. Additionally, the expression levels of HTLV-1 Tax , and HBZ mRNA were quantified via qPCR using PowerUp SYBR Green Master Mix (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (#Hs01042019_g1) and TaqMan® Fast Universal PCR Master Mix (Applied Biosystems; #4352042). ..

    Control:

    Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies
    Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 IPAH PASMC DNA microarray (Affymetrix Human Gene ST 1.0 chip) 25290246 (2015) # Experiment Category Ancestry Diagnosis Age Sex WHO class mPAP (mmHg) CO (L/min) PVR (dyne/sec/cm-5) 1 Cell culture CTRL European Normal 21 M NA NA NA NA 2 Cell culture CTRL European Normal 35 F NA NA NA NA 3* Cell culture CTRL European Normal 50 F NA NA NA NA 4 Cell culture CTRL European Normal 45 M NA NA NA NA 5* Cell culture CTRL European Normal 40 M NA NA NA NA 6* Cell culture CTRL European Normal 53 M NA NA NA NA 7 Cell culture CTRL European Normal 58 M NA NA NA NA 8 Cell culture CTRL European Normal 43 M NA NA NA NA 9* Cell culture CTRL European Normal 56 F NA NA NA NA 10 Cell culture CTRL European Normal 17 F NA NA NA NA 11 Cell culture CTRL European Normal 51 F NA NA NA NA 12 Cell culture CTRL European Normal 12 F NA NA NA NA 13 Cell culture PAH European iPAH 23 F IV Unknown Unknown 720 14* Cell culture PAH European Heritable PAH 61 F III 66 4.5 1012 15 Cell culture PAH European Heritable PAH 62 F Unknown 48 Unknown 640 16* Cell culture PAH European Heritable PAH 35 F IV 60 2.1 1920 17 Cell culture PAH European Heritable PAH 32 F IV 56 5.9 Unknown 18 Cell culture PAH European iPAH 52 M IV 51 5.7 575 19* Cell culture PAH European iPAH 39 M IV Unknown Unknown Unknown 20 Cell culture PAH European iPAH 65 M III 76 4,9 1125 21 Cell culture PAH European SSc-PAH 45 F IV 43 7.25 375 22 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 23* Cell culture PAH European iPAH 52 M IV 68 2.97 Unknown 24 Cell culture PAH European iPAH 59 M IV 66 6.47 630 25 Cell culture PAH European iPAH 40 F Unknown Unknown Unknown Unknown 26* Cell culture PAH European SSc-PAH 62 M IV 55 4.5 816 27 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 28 Cell culture PAH European Heritable PAH 26 F IV 99 Unknown Unknown 29 PCLS CTRL European Tumor resection 69 F NA NA NA NA 30 PCLS CTRL European Tumor resection 69 M NA NA NA NA 31 PCLS CTRL European Tumor resection 67 F NA NA NA NA Table S2. ..

    RNA Sequencing:

    Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies
    Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 IPAH PASMC DNA microarray (Affymetrix Human Gene ST 1.0 chip) 25290246 (2015) # Experiment Category Ancestry Diagnosis Age Sex WHO class mPAP (mmHg) CO (L/min) PVR (dyne/sec/cm-5) 1 Cell culture CTRL European Normal 21 M NA NA NA NA 2 Cell culture CTRL European Normal 35 F NA NA NA NA 3* Cell culture CTRL European Normal 50 F NA NA NA NA 4 Cell culture CTRL European Normal 45 M NA NA NA NA 5* Cell culture CTRL European Normal 40 M NA NA NA NA 6* Cell culture CTRL European Normal 53 M NA NA NA NA 7 Cell culture CTRL European Normal 58 M NA NA NA NA 8 Cell culture CTRL European Normal 43 M NA NA NA NA 9* Cell culture CTRL European Normal 56 F NA NA NA NA 10 Cell culture CTRL European Normal 17 F NA NA NA NA 11 Cell culture CTRL European Normal 51 F NA NA NA NA 12 Cell culture CTRL European Normal 12 F NA NA NA NA 13 Cell culture PAH European iPAH 23 F IV Unknown Unknown 720 14* Cell culture PAH European Heritable PAH 61 F III 66 4.5 1012 15 Cell culture PAH European Heritable PAH 62 F Unknown 48 Unknown 640 16* Cell culture PAH European Heritable PAH 35 F IV 60 2.1 1920 17 Cell culture PAH European Heritable PAH 32 F IV 56 5.9 Unknown 18 Cell culture PAH European iPAH 52 M IV 51 5.7 575 19* Cell culture PAH European iPAH 39 M IV Unknown Unknown Unknown 20 Cell culture PAH European iPAH 65 M III 76 4,9 1125 21 Cell culture PAH European SSc-PAH 45 F IV 43 7.25 375 22 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 23* Cell culture PAH European iPAH 52 M IV 68 2.97 Unknown 24 Cell culture PAH European iPAH 59 M IV 66 6.47 630 25 Cell culture PAH European iPAH 40 F Unknown Unknown Unknown Unknown 26* Cell culture PAH European SSc-PAH 62 M IV 55 4.5 816 27 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 28 Cell culture PAH European Heritable PAH 26 F IV 99 Unknown Unknown 29 PCLS CTRL European Tumor resection 69 F NA NA NA NA 30 PCLS CTRL European Tumor resection 69 M NA NA NA NA 31 PCLS CTRL European Tumor resection 67 F NA NA NA NA Table S2. ..

    Microarray:

    Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies
    Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 IPAH PASMC DNA microarray (Affymetrix Human Gene ST 1.0 chip) 25290246 (2015) # Experiment Category Ancestry Diagnosis Age Sex WHO class mPAP (mmHg) CO (L/min) PVR (dyne/sec/cm-5) 1 Cell culture CTRL European Normal 21 M NA NA NA NA 2 Cell culture CTRL European Normal 35 F NA NA NA NA 3* Cell culture CTRL European Normal 50 F NA NA NA NA 4 Cell culture CTRL European Normal 45 M NA NA NA NA 5* Cell culture CTRL European Normal 40 M NA NA NA NA 6* Cell culture CTRL European Normal 53 M NA NA NA NA 7 Cell culture CTRL European Normal 58 M NA NA NA NA 8 Cell culture CTRL European Normal 43 M NA NA NA NA 9* Cell culture CTRL European Normal 56 F NA NA NA NA 10 Cell culture CTRL European Normal 17 F NA NA NA NA 11 Cell culture CTRL European Normal 51 F NA NA NA NA 12 Cell culture CTRL European Normal 12 F NA NA NA NA 13 Cell culture PAH European iPAH 23 F IV Unknown Unknown 720 14* Cell culture PAH European Heritable PAH 61 F III 66 4.5 1012 15 Cell culture PAH European Heritable PAH 62 F Unknown 48 Unknown 640 16* Cell culture PAH European Heritable PAH 35 F IV 60 2.1 1920 17 Cell culture PAH European Heritable PAH 32 F IV 56 5.9 Unknown 18 Cell culture PAH European iPAH 52 M IV 51 5.7 575 19* Cell culture PAH European iPAH 39 M IV Unknown Unknown Unknown 20 Cell culture PAH European iPAH 65 M III 76 4,9 1125 21 Cell culture PAH European SSc-PAH 45 F IV 43 7.25 375 22 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 23* Cell culture PAH European iPAH 52 M IV 68 2.97 Unknown 24 Cell culture PAH European iPAH 59 M IV 66 6.47 630 25 Cell culture PAH European iPAH 40 F Unknown Unknown Unknown Unknown 26* Cell culture PAH European SSc-PAH 62 M IV 55 4.5 816 27 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 28 Cell culture PAH European Heritable PAH 26 F IV 99 Unknown Unknown 29 PCLS CTRL European Tumor resection 69 F NA NA NA NA 30 PCLS CTRL European Tumor resection 69 M NA NA NA NA 31 PCLS CTRL European Tumor resection 67 F NA NA NA NA Table S2. ..

    Article Title: UV‐A Radiation Impairs Sebaceous‐Gland‐Related Skin Barrier Function by Inducing Inflammation and Altering Intracellular Sebum‐Like Lipid Composition
    Article Snippet: Total RNA was extracted using a NucleoSpin Plus XS kit (Macherey‐Nagel GmbH & Co. KG, Düren, Germany). .. For DNA microarray analysis, 250 ng of total RNA was processed using the Clariom S Array, human (Thermo Fisher Scientific), following the manufacturer's protocol. .. For reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR), total RNA was reverse‐transcribed into cDNA using SuperScript IV VILO Master Mix (Thermo Fisher Scientific), and TaqMan Universal Master Mix II, no UNG (Thermo Fisher Scientific), was used for qPCR reactions.

    Biomarker Discovery:

    Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies
    Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 IPAH PASMC DNA microarray (Affymetrix Human Gene ST 1.0 chip) 25290246 (2015) # Experiment Category Ancestry Diagnosis Age Sex WHO class mPAP (mmHg) CO (L/min) PVR (dyne/sec/cm-5) 1 Cell culture CTRL European Normal 21 M NA NA NA NA 2 Cell culture CTRL European Normal 35 F NA NA NA NA 3* Cell culture CTRL European Normal 50 F NA NA NA NA 4 Cell culture CTRL European Normal 45 M NA NA NA NA 5* Cell culture CTRL European Normal 40 M NA NA NA NA 6* Cell culture CTRL European Normal 53 M NA NA NA NA 7 Cell culture CTRL European Normal 58 M NA NA NA NA 8 Cell culture CTRL European Normal 43 M NA NA NA NA 9* Cell culture CTRL European Normal 56 F NA NA NA NA 10 Cell culture CTRL European Normal 17 F NA NA NA NA 11 Cell culture CTRL European Normal 51 F NA NA NA NA 12 Cell culture CTRL European Normal 12 F NA NA NA NA 13 Cell culture PAH European iPAH 23 F IV Unknown Unknown 720 14* Cell culture PAH European Heritable PAH 61 F III 66 4.5 1012 15 Cell culture PAH European Heritable PAH 62 F Unknown 48 Unknown 640 16* Cell culture PAH European Heritable PAH 35 F IV 60 2.1 1920 17 Cell culture PAH European Heritable PAH 32 F IV 56 5.9 Unknown 18 Cell culture PAH European iPAH 52 M IV 51 5.7 575 19* Cell culture PAH European iPAH 39 M IV Unknown Unknown Unknown 20 Cell culture PAH European iPAH 65 M III 76 4,9 1125 21 Cell culture PAH European SSc-PAH 45 F IV 43 7.25 375 22 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 23* Cell culture PAH European iPAH 52 M IV 68 2.97 Unknown 24 Cell culture PAH European iPAH 59 M IV 66 6.47 630 25 Cell culture PAH European iPAH 40 F Unknown Unknown Unknown Unknown 26* Cell culture PAH European SSc-PAH 62 M IV 55 4.5 816 27 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 28 Cell culture PAH European Heritable PAH 26 F IV 99 Unknown Unknown 29 PCLS CTRL European Tumor resection 69 F NA NA NA NA 30 PCLS CTRL European Tumor resection 69 M NA NA NA NA 31 PCLS CTRL European Tumor resection 67 F NA NA NA NA Table S2. ..

    Cell Culture:

    Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies
    Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 IPAH PASMC DNA microarray (Affymetrix Human Gene ST 1.0 chip) 25290246 (2015) # Experiment Category Ancestry Diagnosis Age Sex WHO class mPAP (mmHg) CO (L/min) PVR (dyne/sec/cm-5) 1 Cell culture CTRL European Normal 21 M NA NA NA NA 2 Cell culture CTRL European Normal 35 F NA NA NA NA 3* Cell culture CTRL European Normal 50 F NA NA NA NA 4 Cell culture CTRL European Normal 45 M NA NA NA NA 5* Cell culture CTRL European Normal 40 M NA NA NA NA 6* Cell culture CTRL European Normal 53 M NA NA NA NA 7 Cell culture CTRL European Normal 58 M NA NA NA NA 8 Cell culture CTRL European Normal 43 M NA NA NA NA 9* Cell culture CTRL European Normal 56 F NA NA NA NA 10 Cell culture CTRL European Normal 17 F NA NA NA NA 11 Cell culture CTRL European Normal 51 F NA NA NA NA 12 Cell culture CTRL European Normal 12 F NA NA NA NA 13 Cell culture PAH European iPAH 23 F IV Unknown Unknown 720 14* Cell culture PAH European Heritable PAH 61 F III 66 4.5 1012 15 Cell culture PAH European Heritable PAH 62 F Unknown 48 Unknown 640 16* Cell culture PAH European Heritable PAH 35 F IV 60 2.1 1920 17 Cell culture PAH European Heritable PAH 32 F IV 56 5.9 Unknown 18 Cell culture PAH European iPAH 52 M IV 51 5.7 575 19* Cell culture PAH European iPAH 39 M IV Unknown Unknown Unknown 20 Cell culture PAH European iPAH 65 M III 76 4,9 1125 21 Cell culture PAH European SSc-PAH 45 F IV 43 7.25 375 22 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 23* Cell culture PAH European iPAH 52 M IV 68 2.97 Unknown 24 Cell culture PAH European iPAH 59 M IV 66 6.47 630 25 Cell culture PAH European iPAH 40 F Unknown Unknown Unknown Unknown 26* Cell culture PAH European SSc-PAH 62 M IV 55 4.5 816 27 Cell culture PAH European iPAH 45 M Unknown Unknown Unknown Unknown 28 Cell culture PAH European Heritable PAH 26 F IV 99 Unknown Unknown 29 PCLS CTRL European Tumor resection 69 F NA NA NA NA 30 PCLS CTRL European Tumor resection 69 M NA NA NA NA 31 PCLS CTRL European Tumor resection 67 F NA NA NA NA Table S2. ..

    TaqMan Copy Number Assay:

    Article Title: Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary‐Patterned Malignancy in Lung Adenocarcinoma
    Article Snippet: .. All MYC copy number detection by laboratory techniques were using MYC copy number TaqMan probe (Thermofiser, # Hs02758348_cn) and TaqMan Copy Number Assay (Thermofisher, #4400291) via manufacturer protocol. ..

    other:

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: TFE3 , Applied Biosystems , Cat.#HS00232406_m1.

    Article Title: RB1 controls differentiation through positive regulation of phosphoglycerate mutases
    Article Snippet: Taqman probes used are as follow: mouse Actb (Mm00607939_s1), mouse Rb (Mm00485586_m1), mouse Hprt (Mm00446968_m1), mouse Nanog (Mm02019550_s1), mouse Pgam2 (Mm01187768_m1), mouse Myh7 (Mm00600555_m1), mouse Myog (Mm00446194_m1), mouse Myod1 (Mm00440387_m1) human ACTB (Hs99999903_m1), human RB (Hs01078066_m1), human PGAM1 (Hs01652468_g1) and human HPRT (Hs02800695_m1).

    Real-time Polymerase Chain Reaction:

    Article Title: Virus-induced RGMa expression drives neurodegeneration in HTLV-1–associated myelopathy
    Article Snippet: The isolated RNA underwent reverse transcriptase (RT) reactions using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO) following the manufacturer’s protocol. .. Real-time qPCR was conducted using ABI Prism 7500 SDS (Applied Biosystems), FastStart Universal Probe Master (ROX) (Roche Diagnostics), and human FAM-labeled TaqMan gene expression primers for the following genes: RGMA (Hs00297192_m1, Applied Biosystems) and Eukaryotic 18S rRNA (4319413E, Applied Biosystems). .. Additionally, the expression levels of HTLV-1 Tax , and HBZ mRNA were quantified via qPCR using PowerUp SYBR Green Master Mix (Applied Biosystems).



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    Image Search Results


    RT q-PCR of a subset of genes from the microarray. Results from RT q-PCR analysis of a subset of up- or down-regulated genes in permanent pulp cells versus deciduous pulp cells. The data agree with the results obtained in the microarray experiments, and were all significantly different, P ≤0.05, Student’s t-test.

    Journal: Stem Cell Research & Therapy

    Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

    doi: 10.1186/s13287-015-0056-7

    Figure Lengend Snippet: RT q-PCR of a subset of genes from the microarray. Results from RT q-PCR analysis of a subset of up- or down-regulated genes in permanent pulp cells versus deciduous pulp cells. The data agree with the results obtained in the microarray experiments, and were all significantly different, P ≤0.05, Student’s t-test.

    Article Snippet: A total of 1 μg RNA for each sample was used for amplification and further analysis with the PIQOR stem cell microarray chip, followed by detection with a laser scanner (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Microarray

    Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec : Pulp cells from deciduous teeth, n = 3 Perm : Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.

    Journal: Stem Cell Research & Therapy

    Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

    doi: 10.1186/s13287-015-0056-7

    Figure Lengend Snippet: Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec : Pulp cells from deciduous teeth, n = 3 Perm : Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.

    Article Snippet: A total of 1 μg RNA for each sample was used for amplification and further analysis with the PIQOR stem cell microarray chip, followed by detection with a laser scanner (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Western Blot, Expressing, Microarray

    RT q-PCR of a subset of genes from the microarray. Results from RT q-PCR analysis of a subset of up- or down-regulated genes in permanent pulp cells versus deciduous pulp cells. The data agree with the results obtained in the microarray experiments, and were all significantly different, P ≤0.05, Student’s t-test.

    Journal: Stem Cell Research & Therapy

    Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

    doi: 10.1186/s13287-015-0056-7

    Figure Lengend Snippet: RT q-PCR of a subset of genes from the microarray. Results from RT q-PCR analysis of a subset of up- or down-regulated genes in permanent pulp cells versus deciduous pulp cells. The data agree with the results obtained in the microarray experiments, and were all significantly different, P ≤0.05, Student’s t-test.

    Article Snippet: Samples were analyzed using a PIQOR Stem Cell Microarray chip (Miltenyi Biotec, Auburn, CA, USA).

    Techniques: Microarray

    Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec : Pulp cells from deciduous teeth, n = 3 Perm : Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.

    Journal: Stem Cell Research & Therapy

    Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

    doi: 10.1186/s13287-015-0056-7

    Figure Lengend Snippet: Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec : Pulp cells from deciduous teeth, n = 3 Perm : Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.

    Article Snippet: Samples were analyzed using a PIQOR Stem Cell Microarray chip (Miltenyi Biotec, Auburn, CA, USA).

    Techniques: Western Blot, Expressing, Microarray