dna microarray analysis nuip on chip cells (Thermo Fisher)
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Dna Microarray Analysis Nuip On Chip Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+microarray+chip/Trypsin/pmc03736349-87-3-19
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Purification:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (# Reverse Transcription:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (# Random Hexamer:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (# Gene Expression:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (# Article Title: Virus-induced RGMa expression drives neurodegeneration in HTLV-1–associated myelopathy Article Snippet: The isolated RNA underwent reverse transcriptase (RT) reactions using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO) following the manufacturer’s protocol. .. Real-time qPCR was conducted using ABI Prism 7500 SDS (Applied Biosystems), FastStart Universal Probe Master (ROX) (Roche Diagnostics), and human FAM-labeled TaqMan gene expression primers for the following genes: RGMA ( Polymerase Chain Reaction:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: As a non-targeted control, siRNA against Firefly luciferase was synthesized (Eurofins Genomics). .. In total, 1 μg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 μl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441), 4 μl 5× reaction buffer, 0.5 μl Random Hexamer Primer, 0.5 mM dNTP mix (10 mM) in a total volume of 20 μl at 25 °C for 10 min, 42 °C for 1 h and 70 °C for 10 min. 1 μl of the reaction mixture was used to amplify cDNA using Taqman® Gene Expression Assays (0.25 μl, (Applied Biosystems) for ACTB (#Hs99999903_m1), GUSB (#Hs99999908_m1), GAPDH (#Hs02758991_g1), IL8 (#Hs00174103_m1), NFKBIA (#Hs00153283_m1), CXCL2 (# Hs00236966_m1), RELA (# Control:Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 RNA Sequencing:Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 Microarray:Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 Article Title: UV‐A Radiation Impairs Sebaceous‐Gland‐Related Skin Barrier Function by Inducing Inflammation and Altering Intracellular Sebum‐Like Lipid Composition Article Snippet: Total RNA was extracted using a NucleoSpin Plus XS kit (Macherey‐Nagel GmbH & Co. KG, Düren, Germany). .. For Biomarker Discovery:Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 Cell Culture:Article Title: Unraveling AURKB as a potential therapeutic target in pulmonary hypertension using integrated transcriptomic analysis and pre-clinical studies Article Snippet: .. GEO series accession number Experimental design Platform PMID (publication year) GSE263226 4 control PASMC vs 5 PAH PASMC (2 IPAH, 2 heritable PAH and 1 SSc-PAH) RNA-Sequencing Present study GSE144274 4 control PASMC vs 4 IPAH PASMC RNA-Sequencing (Illumina HiSeq 4000) 32337710 (2020) Extracted from supplemental data 3 control PASMC vs 3 TaqMan Copy Number Assay:Article Title: Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary‐Patterned Malignancy in Lung Adenocarcinoma Article Snippet: .. All MYC copy number detection by laboratory techniques were using MYC copy number TaqMan probe (Thermofiser, # other:Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA Article Snippet: Article Title: RB1 controls differentiation through positive regulation of phosphoglycerate mutases Article Snippet: Taqman probes used are as follow: mouse Actb (Mm00607939_s1), mouse Rb (Mm00485586_m1), mouse Hprt (Mm00446968_m1), mouse Nanog (Mm02019550_s1), mouse Pgam2 (Mm01187768_m1), mouse Myh7 (Mm00600555_m1), mouse Myog (Mm00446194_m1), mouse Myod1 (Mm00440387_m1) human ACTB (Hs99999903_m1), human RB (Hs01078066_m1), human PGAM1 (Hs01652468_g1) and human HPRT (Hs02800695_m1). Real-time Polymerase Chain Reaction:Article Title: Virus-induced RGMa expression drives neurodegeneration in HTLV-1–associated myelopathy Article Snippet: The isolated RNA underwent reverse transcriptase (RT) reactions using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO) following the manufacturer’s protocol. .. Real-time qPCR was conducted using ABI Prism 7500 SDS (Applied Biosystems), FastStart Universal Probe Master (ROX) (Roche Diagnostics), and human FAM-labeled TaqMan gene expression primers for the following genes: RGMA ( |
